sgRNA
Along with Cas9 nuclease, CRISPR experiments require the introduction of an sgRNA containing an approximately 20 base sequence specific to the target DNA 5’ of a non-variable scaffold sequence. sgRNA can be delivered as RNA or by transforming with a plasmid with the sgRNA-coding sequence under a promoter. A number of strategies have been developed to quickly swap out the 20 base sequences allowing convenient sgRNA cloning using NEB products.